Multisystem combined uranium resistance mechanisms and bioremediation potential of Stenotrophomonas bentonitica BII-R7: Transcriptomics and microscopic study

The potential use of microorganisms in the bioremediation U pollution has been extensively described. However, a lack knowledge on molecular resistance mechanisms become challenge for these technologies. We reported transcriptomic and microscopic response Stenotrophomonas bentonitica BII-R7 exposed to 100 250 ?M U. Results showed that exposure displayed up-regulation 185 148 genes during lag exponential phases, respectively, whereas 143 194 were down-regulated, out 3786 (>1.5-fold change). Exposure 68 down-regulation 290 phase. Genes involved cell wall membrane protein synthesis, efflux systems phosphatases up-regulated under all conditions tested. Microscopic observations evidenced formation U-phosphate minerals at extracellular levels. Thus, biphasic process is likely occur: increased would promote biosorption surface its precipitation as enhanced by phosphatases. Transport prevent accumulation cytoplasm. These findings contribute an understanding how microbes cope with toxicity, thus allowing development efficient strategies.